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| De Conti, Daniela; Corredor-Prado, Jenny Paola; Roecker Junior, Djalma; Suzuki, Rogério Mamoru; Guerra, Miguel Pedro; Pescador, Rosete. |
| Tissue culture techniques have been employed for orchid mass propagation by means of the morphogenetic route of protocorm like-bodies (PLBs). This study aimed to analyze and compare Indole-acetic acid (IAA) and sugar endogenous levels in protocorm-like bodies (PLBs) induction and development in Cattleya tigrina, in order to better understand this process and to optimize micropropagation procedure protocols. Leaves grown on MS (Murashige and Skoog) culture medium, suplemented with 9 μM TDZ for PLBs induction and development were collected after 0, 2, 7, 14, 30, 60 and 100 days of cultivation, for further analysis. Increase of IAA and reduction of sugar levels are strongly related to morphogenetic response, that is, PLBs formation over the preexisting ones... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orchid; Hormones; Sugars; Morphogenesis.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37874 |
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| Oliveira,Luciana do Valle Rego; Faria,Ricardo Tadeu de; Ruas,Claudete de Fátima; Ruas,Paulo Maurício; Santos,Melissa de Oliveira; Carvalho,Valdemar P.. |
| In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catasetum; Molecular marker; Orchid; RAPD; Genetic similarity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200017 |
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| Endres Júnior,Delio; Sasamori,Márcio Hisayuki; Droste,Annette. |
| Anathallis adenochila is a small epiphytic orchid, endemic to the Atlantic Forest in the southern and southeastern regions of Brazil. Information on the species is scarce and limited to distribution and occurrence data, with no reports about its development. In the present study, plantlets were propagated in vitro, and the influence of different concentrations of macronutrient salts and sucrose on the survival and development of the species was assessed. The analysis were performed on complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients) containing 10, 30 or 60 g L-1 of sucrose. Plantlet survival, height of the aerial part, and the length of the longest root were significantly greater on half MS medium containing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Carbon source; Micropropagation; Orchid; Mineral salts. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400001 |
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| Santos,Joice Simone dos; Finger,Fernando Luiz; Mapeli,Ana Maria; Karsten,Juliane; Mendes,Teresa Drummond Correia; Barbosa,José Geraldo. |
| This work had the goal to evaluate the influence of temperature and dry storage on the postharvest longevity of Epidendrum ibaguense flowers previously treated with silver thiosulfate (STS). The flowers were harvested and pulsed for 30 minutes with 2 mM STS. Then both, the STS treated and untreated flowers were packed in perforated low density polyethylene bags, packed in cardboard boxes and stored at 5 and 10°C, where they remained for four and eight days. After this period, the flowers were kept in vase with distilled water at 25ºC, and analyzed at every 24 h. The experiment was arranged in a complete random design 2 x 2 x 2 factorial scheme, with four replicates, containing four inflorescences each, except for the determination of transpiration and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orchid; Cut flower; Temperature; Abscission. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200004 |
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| Pedroso-de-Moraes,Cristiano; Souza-Leal,Thiago de; Brescansin,Rafael L.; Pettini-Benelli,Adarilda; Sajo,Maria das Graças. |
| Considering that the root structure of the Brazilian genera belonging to the Catasetinae subtribe is poorly known, we describe the roots of twelve representatives from this subtribe. For anatomical analysis, the roots were fixed in FAA 50, preserved in ethanol 70% and sectioned at its medium region using razor blades. The sections were stained with 0.05% astra blue and safranin and mounted in glycerin. For the identification of starch we used Lugol's solution; for lignin, floroglucin chloridric; for lipids, Sudan III, and for flavanoids, potassium hydroxide. The relevant aspects were registered using a digital camera joined with an Olympus microspope (BX51 model). The structural similarities of all roots support the placement of the subtribe Catasetinae... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epiphytism; Morphoanatomy; Orchid; Systematic. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200015 |
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| Pedroso-de-Moraes,Cristiano; Souza,Marcelo Claro de; Ronconi,Cínthia Cristina; Marteline,Marco Aurélio. |
| The Cattleya genus has a great importance in the flower agro-business market. Fusarium wilts, caused by Fusarium oxysporum f. sp. cattleyae, is considered one of the main factors of decline and death of plants of this genus. Using seven hybrids (intra and intergenerics) of Cattleya, tests of resistance and susceptibility to F. oxysporum were performed in conditions of greenhouse for 12 months, using, as evaluation criterion, a scale of the disease severity ranging from one (resistant) to eight (highly susceptible). High susceptibility to the fungus by Cattleya Nobile's Wax Toy, Cattleya Orquidacea's Mister Fast intrageneric hybrids and Potinara Orquidacea's Havana Brown intergeneric hybrid, related to Brassocattleya Orquidacea's Melody intergeneric hybrid,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fusarium wilts; Orchid; Varietal resistance. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200007 |
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| Guimarães,Fernanda Aparecida Rodrigues; Pereira,Marlon Corrêa; Felício,Cristiane da Silva; Torres,Denise Pereira; Oliveira,Sabrina Feliciano; Veloso,Tomás Gomes Reis; Kasuya,Maria Catarina Megumi. |
| In nature, orchid seeds obtain the nutrients necessary for germination by degrading intracellular fungal structures formed after colonization of the embryo by mycorrhizal fungi. Protocols for asymbiotic germination of orchid seeds typically use media with high concentrations of soluble carbohydrate and minerals. However, when reintroduced into the field, seedlings obtained via asymbiotic germination have lower survival rates than do seedlings obtained via symbiotic germination. Tree fern fiber, the ideal substrate for orchid seedling acclimatization, is increasingly scarce. Here, we evaluated seed germination and protocorm development of Cyrtopodium glutiniferum Raddi cultivated in asymbiotic media (Knudson C and Murashige & Skoog) and in oatmeal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epulorhiza sp.; Symbiosis; Orchid; Substrate; Fern fiber. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000300016 |
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